| Description: | The updated RIDA® QUICK (N1402) immunochromatographic assay (R-Biopharm) for detection of norovirus
was evaluated during a prospective, multicenter study using 771 stool samples from patients with gastroenteritis.
Compared to real-time reverse transcriptase polymerase chain reaction (RT-rtPCR) as gold standard, the
RIDA®QUICK had an overall sensitivity of 72.8% (91/125) and a specificity of 99.5% (640/643). Genotype analysis
of the polymerase (ORF1) and capsid (ORF2) region of the genome indicated that the RIDA® QUICK assay could
detect a broad range of genotypes including new variants (15 of 125 positive samples) which were detected by
an in-house SYBR®Green RT-rtPCR, but not by the RIDA®GENE PCR PG1415 (R-Biopharm) andmostly not by the
RIDA® GENE PCR PG1405 and the Xpert® Norovirus assay (Cepheid). The RIDA® QUICK can be used to reliably
confirm norovirus in stool samples, but a negative result does not definitively exclude the presence of norovirus. |